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eclipse ti e c2 confocal laser scanning microscope  (Nikon)


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    Structured Review

    Nikon eclipse ti e c2 confocal laser scanning microscope
    Colony and biofilm calcofluor staining assays. Production of exopolysaccharide (EPS) by the wild-type ( A. brasilense Sp7), the cysK-A mutant ( A. brasilense AR), and the complemented ( A . brasilense AR-pAB- cysK ) strains were visualized by staining with calcofluor, which binds to β-linked polysaccharides. ( A ) Colonies were grown for 5 days at 30 °C on solid LB medium (lacking NaCl) supplemented with Calcofluor (200 μg/mL). Images were captured under UV illumination to visualize fluorescence. Scale bar = 10 mm. ( B ) Confocal micrographs of biofilms grown for 5 days at 30 °C on agar-solidified Nfb∗ medium containing Calcofluor (85 μM). The biofilms were observed with a Nikon <t>Eclipse</t> <t>Ti-E</t> <t>C2+</t> <t>microscope.</t> Scale bar = 20 μm.
    Eclipse Ti E C2 Confocal Laser Scanning Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ti+e+confocal+microscope/C2%2B/pmc12811640-78-16-23
    Average 99 stars, based on 1 article reviews
    eclipse ti e c2 confocal laser scanning microscope - by Bioz Stars, 2026-09
    99/100 stars

    Images

    1) Product Images from "Inactivation of Cysteine Synthase CysK-A enhances flocculation, biofilm formation, and sensitivity to oxidative stress in Azospirillum brasilense Sp7"

    Article Title: Inactivation of Cysteine Synthase CysK-A enhances flocculation, biofilm formation, and sensitivity to oxidative stress in Azospirillum brasilense Sp7

    Journal: Biofilm

    doi: 10.1016/j.bioflm.2025.100335

    Colony and biofilm calcofluor staining assays. Production of exopolysaccharide (EPS) by the wild-type ( A. brasilense Sp7), the cysK-A mutant ( A. brasilense AR), and the complemented ( A . brasilense AR-pAB- cysK ) strains were visualized by staining with calcofluor, which binds to β-linked polysaccharides. ( A ) Colonies were grown for 5 days at 30 °C on solid LB medium (lacking NaCl) supplemented with Calcofluor (200 μg/mL). Images were captured under UV illumination to visualize fluorescence. Scale bar = 10 mm. ( B ) Confocal micrographs of biofilms grown for 5 days at 30 °C on agar-solidified Nfb∗ medium containing Calcofluor (85 μM). The biofilms were observed with a Nikon Eclipse Ti-E C2+ microscope. Scale bar = 20 μm.
    Figure Legend Snippet: Colony and biofilm calcofluor staining assays. Production of exopolysaccharide (EPS) by the wild-type ( A. brasilense Sp7), the cysK-A mutant ( A. brasilense AR), and the complemented ( A . brasilense AR-pAB- cysK ) strains were visualized by staining with calcofluor, which binds to β-linked polysaccharides. ( A ) Colonies were grown for 5 days at 30 °C on solid LB medium (lacking NaCl) supplemented with Calcofluor (200 μg/mL). Images were captured under UV illumination to visualize fluorescence. Scale bar = 10 mm. ( B ) Confocal micrographs of biofilms grown for 5 days at 30 °C on agar-solidified Nfb∗ medium containing Calcofluor (85 μM). The biofilms were observed with a Nikon Eclipse Ti-E C2+ microscope. Scale bar = 20 μm.

    Techniques Used: Staining, Mutagenesis, Fluorescence, Microscopy

    Related Articles

    Microscopy:

    Article Title: The Antagonizing Role of Heme in the Antimalarial Function of Artemisinin: Elevating Intracellular Free Heme Negatively Impacts Artemisinin Activity in Plasmodium falciparum
    Article Snippet: .. ZnMP uptake was examined under a Nikon Ti-E confocal microscope equipped with a ×100 MPLAPON oil objective with the excitation wavelength at 488 nm and the emission spectrum at 550–650 nm. .. Arabidopsis heme oxygenase gene (AtHO) was inserted into pCC4 plasmid, a vector with a blasticidin (BSD) selectable marker.

    Article Title: Iron nanoparticle coating and plasma-activated water cleaning as a biofouling removal method for reverse osmosis membranes suitable for desalination
    Article Snippet: .. Following this, the stained membrane was transferred to a 6-well glass bottom well plate (P06-20-1-N, Cellvis) and imaged using a Nikon Ti-E confocal microscope with 20× objective and imaged at the Excitation/Emission wavelengths (Ex/Em) of 480/500 nm for Syto9, and 490/635 nm for PI, respectively. .. The fluorescent images were analysed via NIS element software (Nikon).

    Article Title: Host transcription factor Speckled 110 kDa (Sp110), a nuclear body protein, is hijacked by hepatitis B virus protein X for viral persistence
    Article Snippet: Flow cytometric data acquisition was performed on BD FACSCalibur platform. .. Finally, the imaging was done with Nikon Ti-E confocal microscope with A1RMP scanner head and Zeiss LSM 510 meta confocal microscope. ..

    Article Title: Host transcription factor Speckled 110 kDa (Sp110), a nuclear body protein, is hijacked by hepatitis B virus protein X for viral persistence
    Article Snippet: .. Finally, the imaging was done with Nikon Ti-E confocal microscope with A1RMP scanner head and Zeiss LSM 510 Meta Confocal Microscope. ..

    Article Title: Sirtuin 2 promotes human cytomegalovirus replication by regulating cell cycle progression.
    Article Snippet: The cells were then washed with 0.2% PBS-T prior to mounting with ProLong Diamond anti-fade (ThermoFisher). .. Immunofluorescence microscopy analysis was conducted using a Nikon Ti-E confocal microscope equipped with a spinning disc module. ..

    Article Title: TCF19 Promotes Cell Proliferation through Binding to the Histone H3K4me3 Mark.
    Article Snippet: Subscriber access provided by UNIVERSITY OF CONNECTICUT is published by the American Chemical Society.. 1155 Sixteenth Street N.W., Washington, DC 20036 Published by American Chemical Society.. Copyright © American Chemical Society.

    Article Title: Sirtuin 2 promotes human cytomegalovirus replication by regulating cell cycle progression
    Article Snippet: The cells were then washed with 0.2% PBS-T prior to mounting with ProLong Diamond anti-fade (ThermoFisher). .. Immunofluorescence microscopy analysis was conducted using a Nikon Ti-E confocal microscope equipped with a spinning disc module. ..

    Staining:

    Article Title: Iron nanoparticle coating and plasma-activated water cleaning as a biofouling removal method for reverse osmosis membranes suitable for desalination
    Article Snippet: .. Following this, the stained membrane was transferred to a 6-well glass bottom well plate (P06-20-1-N, Cellvis) and imaged using a Nikon Ti-E confocal microscope with 20× objective and imaged at the Excitation/Emission wavelengths (Ex/Em) of 480/500 nm for Syto9, and 490/635 nm for PI, respectively. .. The fluorescent images were analysed via NIS element software (Nikon).

    Membrane:

    Article Title: Iron nanoparticle coating and plasma-activated water cleaning as a biofouling removal method for reverse osmosis membranes suitable for desalination
    Article Snippet: .. Following this, the stained membrane was transferred to a 6-well glass bottom well plate (P06-20-1-N, Cellvis) and imaged using a Nikon Ti-E confocal microscope with 20× objective and imaged at the Excitation/Emission wavelengths (Ex/Em) of 480/500 nm for Syto9, and 490/635 nm for PI, respectively. .. The fluorescent images were analysed via NIS element software (Nikon).

    Imaging:

    Article Title: Host transcription factor Speckled 110 kDa (Sp110), a nuclear body protein, is hijacked by hepatitis B virus protein X for viral persistence
    Article Snippet: Flow cytometric data acquisition was performed on BD FACSCalibur platform. .. Finally, the imaging was done with Nikon Ti-E confocal microscope with A1RMP scanner head and Zeiss LSM 510 meta confocal microscope. ..

    Article Title: Host transcription factor Speckled 110 kDa (Sp110), a nuclear body protein, is hijacked by hepatitis B virus protein X for viral persistence
    Article Snippet: .. Finally, the imaging was done with Nikon Ti-E confocal microscope with A1RMP scanner head and Zeiss LSM 510 Meta Confocal Microscope. ..

    Article Title: TCF19 Promotes Cell Proliferation through Binding to the Histone H3K4me3 Mark.
    Article Snippet: Subscriber access provided by UNIVERSITY OF CONNECTICUT is published by the American Chemical Society.. 1155 Sixteenth Street N.W., Washington, DC 20036 Published by American Chemical Society.. Copyright © American Chemical Society.

    Immunofluorescence:

    Article Title: Sirtuin 2 promotes human cytomegalovirus replication by regulating cell cycle progression.
    Article Snippet: The cells were then washed with 0.2% PBS-T prior to mounting with ProLong Diamond anti-fade (ThermoFisher). .. Immunofluorescence microscopy analysis was conducted using a Nikon Ti-E confocal microscope equipped with a spinning disc module. ..

    Article Title: Sirtuin 2 promotes human cytomegalovirus replication by regulating cell cycle progression
    Article Snippet: The cells were then washed with 0.2% PBS-T prior to mounting with ProLong Diamond anti-fade (ThermoFisher). .. Immunofluorescence microscopy analysis was conducted using a Nikon Ti-E confocal microscope equipped with a spinning disc module. ..



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    Colony and biofilm calcofluor staining assays. Production of exopolysaccharide (EPS) by the wild-type ( A. brasilense Sp7), the cysK-A mutant ( A. brasilense AR), and the complemented ( A . brasilense AR-pAB- cysK ) strains were visualized by staining with calcofluor, which binds to β-linked polysaccharides. ( A ) Colonies were grown for 5 days at 30 °C on solid LB medium (lacking NaCl) supplemented with Calcofluor (200 μg/mL). Images were captured under UV illumination to visualize fluorescence. Scale bar = 10 mm. ( B ) Confocal micrographs of biofilms grown for 5 days at 30 °C on agar-solidified Nfb∗ medium containing Calcofluor (85 μM). The biofilms were observed with a Nikon <t>Eclipse</t> <t>Ti-E</t> <t>C2+</t> <t>microscope.</t> Scale bar = 20 μm.
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    Image Search Results


    Colony and biofilm calcofluor staining assays. Production of exopolysaccharide (EPS) by the wild-type ( A. brasilense Sp7), the cysK-A mutant ( A. brasilense AR), and the complemented ( A . brasilense AR-pAB- cysK ) strains were visualized by staining with calcofluor, which binds to β-linked polysaccharides. ( A ) Colonies were grown for 5 days at 30 °C on solid LB medium (lacking NaCl) supplemented with Calcofluor (200 μg/mL). Images were captured under UV illumination to visualize fluorescence. Scale bar = 10 mm. ( B ) Confocal micrographs of biofilms grown for 5 days at 30 °C on agar-solidified Nfb∗ medium containing Calcofluor (85 μM). The biofilms were observed with a Nikon Eclipse Ti-E C2+ microscope. Scale bar = 20 μm.

    Journal: Biofilm

    Article Title: Inactivation of Cysteine Synthase CysK-A enhances flocculation, biofilm formation, and sensitivity to oxidative stress in Azospirillum brasilense Sp7

    doi: 10.1016/j.bioflm.2025.100335

    Figure Lengend Snippet: Colony and biofilm calcofluor staining assays. Production of exopolysaccharide (EPS) by the wild-type ( A. brasilense Sp7), the cysK-A mutant ( A. brasilense AR), and the complemented ( A . brasilense AR-pAB- cysK ) strains were visualized by staining with calcofluor, which binds to β-linked polysaccharides. ( A ) Colonies were grown for 5 days at 30 °C on solid LB medium (lacking NaCl) supplemented with Calcofluor (200 μg/mL). Images were captured under UV illumination to visualize fluorescence. Scale bar = 10 mm. ( B ) Confocal micrographs of biofilms grown for 5 days at 30 °C on agar-solidified Nfb∗ medium containing Calcofluor (85 μM). The biofilms were observed with a Nikon Eclipse Ti-E C2+ microscope. Scale bar = 20 μm.

    Article Snippet: After five days of static incubation at 30 °C, three-dimensional biofilm structures were observed using an Eclipse Ti-E C2+ confocal laser scanning microscope (Nikon) equipped with CFI Plan Apo VC 20x/1.2 and CFI Plan Apo VC 60x/1.2 WI objective lenses.

    Techniques: Staining, Mutagenesis, Fluorescence, Microscopy